PeptideCalculations
coenzyme, not a peptide

NAD+ dosage

NAD+ is a coenzyme, not a peptide, sold in 100 to 1,000 mg vials. Clinics run it IV at 250 to 1,000 mg over several hours; the one published human infusion study used 750 mg over 6 hours. Subcutaneous use is 50 to 100 mg per shot, one to three times a week, sometimes daily. Inject slowly. Fast delivery causes flushing, chest tightness and nausea.

By the Peptide Calculations editors · Updated 2026-10-19 · Studied for energy and fatigue, addiction recovery clinics, aging markers

Dose
750 mg over 6 hours IV
per infusion · Human trial
How often
subcutaneous: 1 to 3 times a week
morning or midday; it can be stimulating
How long
clinic courses run 4 to 6 weeks
subcutaneous use is often ongoing at 1 to 2 shots a week
Route
subcutaneous
abdomen or IV in a clinic; inject subcutaneous doses slowly over a minute or more

1Dose ranges by source

RangePerBasisNote
750 mg over 6 hours IVinfusionHuman trial
clinical trial (Grant et al. 2019, Frontiers in Aging Neuroscience, 8 men, pharmacokinetic pilot)
about 2 mg per minute; the authors chose it because it matched a common clinic regimen; no adverse events
250 mg to 1,000 mg IVinfusionCommonly reported
commonly reported
clinic IV drips over 2 to 4 hours, 1 to 5 times a week in short courses
50 mg to 100 mg subcutaneousinjectionCommonly reported
commonly reported
1 to 3 times a week, some daily; a registered safety study (NCT07251608) uses the same 50 mg or 100 mg subcutaneous or intramuscular doses

2Dose to units chart

500 mg vial + 5 mL bacteriostatic water = 100 mg/mL, so each unit on a U-100 insulin syringe holds 1,000 mcg.

DoseDraw (mL)UnitsDoses in vial
750 mg7.57500
1,000 mg101,0000
Open in the NAD+ calculatorFull 500 mg + 5 mL table

500 mg + 5 mL = 100 mg/mL, so 100 mg = 1 mL = 100 units (a full insulin syringe) and 50 mg = 50 units. Large doses mean large volumes; split a 100 mg shot across two sites if it stings.

3Side effects

flushingchest tightness or pressure during fast deliverynauseainjection site stingingheadachecramping
Before you start. The only published direct infusion study is a pharmacokinetic pilot in 11 men; no trial shows a clinical benefit from injected NAD+. Nearly all dosing comes from clinics. People with heart rhythm problems should not do fast or large doses. Not medical advice. Talk to a clinician and start low.

Storage: lyophilized: fridge, protect from light; reconstituted: fridge, use within 2 to 4 weeks (commonly reported); it degrades faster than peptides

4NAD+ dosage questions

How much NAD+ to inject subcutaneously?

50 to 100 mg per shot, one to three times a week, is the commonly reported range and the range a registered safety study is testing. At 100 mg/mL that is 50 to 100 units. Start at 50 mg and inject slowly.

How much NAD+ is in an IV drip?

Clinics run 250 to 1,000 mg over 2 to 4 hours. The published human pilot used 750 mg over 6 hours at about 2 mg per minute, with no adverse events in 8 men. Faster rates are what cause chest pressure and nausea.

Can you take NAD+ every day?

Some people do 50 mg daily subcutaneously and some clinics do 5 IV sessions a week for 4 to 6 weeks. No trial has compared schedules. Most ongoing users settle at 1 to 2 shots a week.

Why does NAD+ injection burn or cause flushing?

NAD+ is a vasodilator and the solution is acidic at high concentration. Injecting slowly, using a lower concentration (more water) and splitting the dose between two sites cuts both the sting and the flush.

5Sources

  1. Grant R et al., Front Aging Neurosci 2019, A pilot study of plasma and urine NAD+ metabolome during a 6 hour intravenous infusion of NAD+ (750 mg)
  2. ClinicalTrials.gov NCT07251608, safety study of subcutaneous and intramuscular NAD+ injections (50 mg or 100 mg)
  3. Peptide Protocol Wiki, NAD+ 100 mg dosing calculator (community subcutaneous ranges)

Educational summary of published labels, trials and commonly reported use. Not medical advice.

Brands we recommend for NAD+Independent. No affiliation with any brand, no affiliate links, no referral codes, no paid placements.
  1. 1Licensed Peptides two accredited labs on every lot, certificates public, card and Apple Pay checkout
  2. 2Core Peptides long track record, public certificates, wide catalog
  3. 3Ascension Peptides public third-party testing, clear pricing