PeptideCalculations

How much water to add

Work backwards. Choose the concentration you want, or the unit count you want a given amount to land on, and get the millilitres to add.

mg ÷ target concentration

units
Add
—mL
Resulting concentration
—mg/mL

Volume table

Vial1 mL2 mL3 mL5 mL
2 mg2 mg/mL · 20 mcg/unit1 mg/mL · 10 mcg/unit0.67 mg/mL · 6.7 mcg/unit0.4 mg/mL · 4 mcg/unit
5 mg5 mg/mL · 50 mcg/unit2.5 mg/mL · 25 mcg/unit1.67 mg/mL · 16.7 mcg/unit1 mg/mL · 10 mcg/unit
10 mg10 mg/mL · 100 mcg/unit5 mg/mL · 50 mcg/unit3.33 mg/mL · 33.3 mcg/unit2 mg/mL · 20 mcg/unit
15 mg15 mg/mL · 150 mcg/unit7.5 mg/mL · 75 mcg/unit5 mg/mL · 50 mcg/unit3 mg/mL · 30 mcg/unit
20 mg20 mg/mL · 200 mcg/unit10 mg/mL · 100 mcg/unit6.67 mg/mL · 66.7 mcg/unit4 mg/mL · 40 mcg/unit
30 mg30 mg/mL · 300 mcg/unit15 mg/mL · 150 mcg/unit10 mg/mL · 100 mcg/unit6 mg/mL · 60 mcg/unit
50 mg50 mg/mL · 500 mcg/unit25 mg/mL · 250 mcg/unit16.67 mg/mL · 166.7 mcg/unit10 mg/mL · 100 mcg/unit
60 mg60 mg/mL · 600 mcg/unit30 mg/mL · 300 mcg/unit20 mg/mL · 200 mcg/unit12 mg/mL · 120 mcg/unit

The volume is a choice

Nothing about the vial dictates the volume. The mass on the label is fixed; the solvent sets how that mass is spread out. Less solvent means a higher concentration and fewer units per draw, which is easier to fit in a small barrel but harder to read precisely. More solvent means more units per draw, easier to read, but a 1 mL barrel runs out sooner. The water guide explains how to pick a volume that gives round numbers, and the vial tables show every pairing.

Questions

How much bacteriostatic water should I add to a 5 mg vial?

Any volume the vial holds; the choice sets the concentration, not the mass. 1 mL gives 5 mg/mL, 2 mL gives 2.5 mg/mL, 3 mL gives 1.67 mg/mL. Pick the volume that puts your amount per draw on a round, readable unit count.

Can I add more water later?

Arithmetically yes: recompute the concentration with the total volume. 5 mg in 2 mL plus 1 mL more is 5 mg in 3 mL. Whether a vial should be topped up is not a math question and this site does not address it.

What is bacteriostatic water?

Sterile water containing 0.9% benzyl alcohol, sold in multi-use vials. It is the solvent most lyophilized peptide labels name. The math is identical for any solvent.

What if the volume I need does not fit the vial?

Most lyophilized vials are 2 mL or 3 mL glass. If the calculator asks for more than the vial holds, accept a higher concentration (fewer units per draw) or use a larger barrel.

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